From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila

A monoclonal antibody (MAb24B10), derived from mice immunized with Drosophila retina, exclusively stains photoreceptor cells in the retina and their axonal projections to the optic ganglia. The antigen (Ag24B10) is a 160-kDa glycoprotein comprising about 0.8% of the retina protein. By microsequencing, 19 of the first 21 amino acids at the NH2-terminal end of the protein have been determined. Using synthetic oligonucleotide probes corresponding to a portion of this amino acid sequence, we isolated a homologous genomic clone. A partial DNA sequence of this clone, along with blot experiments on genomic DNA and RNA, indicate that this clone is part of the structural gene for Ag24B10. By in situ hybridization, the gene was localized to the tip of chromosome 3R

Breeding properties of Tinca tinca (L., 1758) living in Kapulukaya Reservoir (Kirikkale, Turkey)

This study involves the investigation of Tinca tinca (L., 1758) living in Kapulukaya Reservoir in Turkey. It was observed that the fish reach sexual maturity at the age of III and the breeding period starts in May and ends in July. The mean monthly gonadosomatic indices varied from 1.38 ±0.06 (in October) to 8.01±1.03 (in May). According to monthly variations of the GSI, spawning of the tench takes place between May (8.01 ± 1.03) and June (5.50 ± 0.57). The average egg number per fish was estimated to be 23403 ± 17047 . The eggs diameters varied from 0.50 mm to 1.33 mm (mean 0.86 mm). The relations between the fecundity (F) and body length (L) and body weight (W) were found as F = 0.037799 × L 1.0212 (r = 0.70) and F = 0. 00000175 × W 2.8696 (r = 0.62)

Short communication: Growth properties of tench (Tinca tinca L., 1758) living in Hirfanlı Reservoir (Kırşehir, Turkey)

Tinca tinca is originally from the Near East and West-Siberia and nowadays is spread all over the world. Previous reports showed that they abundantly present in the rivers flowing to the Black Sea and various lakes as well as in rivers in Central Anatolia. It has no economical importance due to its slow growth rate and tasteless meat. There are many publications on the growth and the bio-ecology and feeding of T. tinca. This study was carried out to investigate the growth properties, such as weight, length and condition factor, in T. tinca inhabit in Hirfanlı Reservoir built in 1959 on Kızılırmak River with the altitude of 856m and volume of 7.63 x10^9m^3. The study was carried out from August 1996 to July 1997 on 241 T. tinca species

Electron Tomographic Studies of Mitochondrial Crista Topology: “Swirl” Mitochondria of Drosophila Flight Muscle

Extended abstract of a paper presented at Microscopy and Microanalysis 2007 in Ft. Lauderdale, Florida, USA, August 5 – August 9, 2007

Bounded Representations of Interval and Proper Interval Graphs

Klavik et al. [arXiv:1207.6960] recently introduced a generalization of recognition called the bounded representation problem which we study for the classes of interval and proper interval graphs. The input gives a graph G and in addition for each vertex v two intervals L_v and R_v called bounds. We ask whether there exists a bounded representation in which each interval I_v has its left endpoint in L_v and its right endpoint in R_v. We show that the problem can be solved in linear time for interval graphs and in quadratic time for proper interval graphs. Robert's Theorem states that the classes of proper interval graphs and unit interval graphs are equal. Surprisingly the bounded representation problem is polynomially solvable for proper interval graphs and NP-complete for unit interval graphs [Klav\'{\i}k et al., arxiv:1207.6960]. So unless P = NP, the proper and unit interval representations behave very differently. The bounded representation problem belongs to a wider class of restricted representation problems. These problems are generalizations of the well-understood recognition problem, and they ask whether there exists a representation of G satisfying some additional constraints. The bounded representation problems generalize many of these problems

Minimal Obstructions for Partial Representations of Interval Graphs

Interval graphs are intersection graphs of closed intervals. A generalization of recognition called partial representation extension was introduced recently. The input gives an interval graph with a partial representation specifying some pre-drawn intervals. We ask whether the remaining intervals can be added to create an extending representation. Two linear-time algorithms are known for solving this problem. In this paper, we characterize the minimal obstructions which make partial representations non-extendible. This generalizes Lekkerkerker and Boland's characterization of the minimal forbidden induced subgraphs of interval graphs. Each minimal obstruction consists of a forbidden induced subgraph together with at most four pre-drawn intervals. A Helly-type result follows: A partial representation is extendible if and only if every quadruple of pre-drawn intervals is extendible by itself. Our characterization leads to a linear-time certifying algorithm for partial representation extension

Light Activation of an Innate Olfactory Avoidance Response in Drosophila

How specific sensory stimuli evoke specific behaviors is a fundamental problem in neurobiology. In Drosophila, most odorants elicit attraction or avoidance depending on their concentration, as well as their identity [1]. Such odorants, moreover, typically activate combinations of glomeruli in the antennal lobe of the brain 2, 3 and 4, complicating the dissection of the circuits translating odor recognition into behavior. Carbon dioxide (CO_2), in contrast, elicits avoidance over a wide range of concentrations 5 and 6 and activates only a single glomerulus, V [5]. The V glomerulus receives projections from olfactory receptor neurons (ORNs) that coexpress two GPCRs, Gr21a and Gr63a, that together comprise a CO_2 receptor 7, 8 and 9. These CO_2-sensitive ORNs, located in the ab1 sensilla of the antenna, are called ab1c neurons [10]. Genetic silencing of ab1c neurons indicates that they are necessary for CO_2-avoidance behavior [5]. Whether activation of these neurons alone is sufficient to elicit this behavior, or whether CO_2 avoidance requires additional inputs (e.g., from the respiratory system), remains unclear. Here, we show that artificial stimulation of ab1c neurons with light (normally attractive to flies) elicits the avoidance behavior typical of CO_2. Thus, avoidance behavior appears hardwired into the olfactory circuitry that detects CO_2 in Drosophila

Human ApoD, an apolipoprotein up-regulated in neurodegenerative diseases, extends lifespan and increases stress resistance in Drosophila

Apolipoprotein D (ApoD) expression increases in several neurological disorders and in spinal cord injury. We provide a report of a physiological role for human ApoD (hApoD): Flies overexpressing hApoD are long-lived and protected against stress conditions associated with aging and neurodegeneration, including hyperoxia, dietary paraquat, and heat stress. We show that the fly ortholog, Glial Lazarillo, is strongly up-regulated in response to these extrinsic stresses and also can protect in vitro-cultured cells in situations modeling Alzheimer's disease (AD) and Parkinson's disease (PD). In adult flies, hApoD overexpression reduces age-associated lipid peroxide accumulation, suggesting a proximal mechanism of action. Similar data obtained in the mouse [Ganfornina, M.D., et al., (2008) Apolipoprotein D is involved in the mechanisms regulating protection from oxidative stress. Aging Cell 10.1111/j.1474-9726.2008.00395.] as well as in plants (Charron et al., personal communication) suggest that ApoD and its orthologs play an evolutionarily conserved role in response to stress, possibly managing or preventing lipid peroxidation